Protein Purification
Gold Biotechnology Inc Nickel NTA Agarose Beads 100 mL
Nickel-NTA Agarose Resin has a binding capacity of ~50 mg/ml. In addition, it allows for purification of proteins under native or denaturing conditions. GoldBio Nickel Agarose works very well with the His-Tag Buffer Set.
Dojindo Molecular Technologies Inc Dojindo ExoSparkler Exosome Protein Labeling Kit, Red, 1 to 10 μg Protein per Sample, 5 Samples
This kit allows for exosome protein fluorescent staining and enables observation of exosome dynamics without extracellular aggregation, covering steps from fluorescence labeling to purification. It can be used for staining purified exosomal membrane or protein, allowing imaging of labeled exosomes taken up by cells.
- Enables observation of exosome dynamics without extracellular aggregation.
- Includes filtration tubes for removal of unreacted dyes.
- Optimized protocol for exosome labeling.
- Recommended protein amount per sample: 1 to 10 μg.
- Recommended particle count per sample: 10 to 100 x 108 particles.
- Detection conditions (red): Ex 561 nm/Em 570 to 640 nm.
- Recommended purification methods include ultracentrifugation, immunoprecipitation, and magnetic beads.
- Store at 0°C to 5°C, protected from moisture.
Zymo Research Corporation Zymo-Spin™ IIICG Columns (50 Pack) (Capped) Green Retension Ring
The Zymo-Spin IIICG features durable polypropylene construction and contains a unique silica-based matrix that allows purification of up to 25 g of DNA, and 100 g of RNA, in 50l eluate. Capacity is 800 l.
IBA LifeSciences Twin-Strep-tag Capture Kit for SPR analysis; Classic size
Twin-Strep-tag Capture Kit is intended for site-directed, reversible capture of Twin-Strep-tag fusion proteins for biomolecular interaction analysis using surface plasmon resonance (SPR) devices. The kit provides Strep-TactinXT, SPR Immobilization Buffer, Regeneration Buffer, and GFP-Twin-Strep-tag as control protein. It enables the highly stable immobilization of the ligand based on the unique Strep-TactinXT:Twin-Strep-tag interaction, which operates with pM affinity. The key benefits are:• Indirect immobilization protecting the ligand from chemical modification as well as inactivation or manipulation by the immobilization procedure• High affinity enabling measurements over a long period• Chip can be regenerated easily by an established procedure• Ligands can be captured directly from complex samples due to the high affinity and specificityThe use of unconjugated Strep-TactinXT requires a license that is provided with the purchase of the kit as limited use license.
Showa Denko America Inc SHODEX OHPAK SB-807 HQ COLUMN
Polyhydroxymethacrylate columns for aqueous SEC (GFC) analysis. The eluent can be replaced with DMF for the analysis of polar polymers. Good replacement for protein analysis when silica cannot be used. Pullulan exclusion limit: 500,000,000
STEMCELL Technologies EasySep™ Direct Human T Cell Isolation Kit, For processing: 100 mL whole blood processed
This cell separation kit can be used to isolate highly purified T cells directly from human whole blood by immunomagnetic negative selection.This kit targets non-T cells for removal with antibodies recognizing specific surface markers. Unwanted cells are labeled with antibodies and EasySep™ Direct RapidSpheres™, and separated using an EasySep™ magnet. Desired cells are simply collected into a new tube and can directly be used for functional assays, flow cytometry or DNA/RNA extraction.
Biotage GEL FILTRATION PHYTIP COLUMNS
Gel filtration PhyTip® columns (1000 µL volume) containing 600 µL of 5K size exclusion resin per column
Sigma Aldrich Fine Chemicals Biosciences cOmplete(TM), EDTA-free Protease Inhibitor Cocktail Tablets provided in glass vials |
cOmplete(TM), EDTA-free Protease Inhibitor Cocktail Tablets provided in glass vials |
Sigma Aldrich Fine Chemicals Biosciences Nuclei Isolation Kit: Nuclei EZ Prep
The Nuclei EZ Prep Kit was developed for the rapid isolation of nuclei from mammalian cells. The kit provides a high yield of nuclei from commonly used cell types. Suitable as a source of nuclear components, to produce nuclei for in vitro apoptosis assays, and for functional studies.
Supply Solutions Protease Inhibitor Cocktail, MFCD00677817, 1mL
For use with mammalian cell and tissue extracts, DMSO solution, Inhibits serine, cysteine, and acid proteases, and aminopeptidases.
Sigma Aldrich Fine Chemicals Biosciences Anti-FLAG(R) M2 Magnetic Beads affinity isolated antibody | 5ML
Anti-FLAG(R) M2 Magnetic Beads affinity isolated antibody | 5ML
STEMCELL Technologies EasySep™ Human B Cell Isolation Kit, For processing: 1 x 10^9 cells
The EasySep™ Human B Cell Isolation Kit is designed to isolate B cells from fresh or previously frozen peripheral blood mononuclear cells or washed leukapheresis samples by immunomagnetic negative selection. The EasySep™ procedure involves labeling unwanted cells with antibody complexes and magnetic particles. The magnetically labeled cells are separated from the untouched, desired cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube. This product can be used in place of the EasySep™ Human B Cell Enrichment Kit (Catalog #19054) for even faster cell isolations.
Sigma Aldrich Fine Chemicals Biosciences Triton(TM) X-100 laboratory grade | 9002-93-1 | MFCD00128254 | 1L
Triton(TM) X-100 laboratory grade | 9002-93-1 | MFCD00128254 | 1L